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Tissue Culture Propagation
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Posted by judith_sydney NSW (My Page) on Sun, Jan 15, 06 at 23:58
Hi all,
Has anybody here had any success with Tissue Culture Propogation at home? (rather than in the Lab).
I find the subject fascinating and am about to attempt my own home tissue culture experiment.
Martin |
Follow-Up Postings:
RE: Tissue Culture Propagation
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| Martin, I have done LOTS of tissue culture at work (in a lab) and it's quite tricky! I wish you all the best with your experiments. My advice is to get yourself a large pressure cooker and cook all your media/equipment in that. You need at least 20 minutes to ensure all microbes are destroyed. Wrap everything in aluminium foil before sterilising. The next hurdle is sorting out some kind of laminar flow cabinet. Might cost you a bit but I’m sure it can be done. If you need any advice regarding media, equipment, techniques etc I’m more than happy to help. Cheers, Jamus. |
RE: Tissue Culture Propagation
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Hey Jamus,
Thanks for your reply.
I have read a bit about it and find the subject really interesting.
There is a fair bit of info around on doing it at home, using pressure cookers etc to sterilise, and saying just put away from direct sun at room temperature.
I have just set some up the other day, whether they are sterile enough, I would say possibly not, however they have been in the jars 2 days with no sign of shrivelling up or any mould or anything, so all I can do is cross my fingers.
I don't expect 100% success rate in a crewd home setup, but if I get a couple of tips shooting that survive, I'll be utterly stoked!
Will pop you a line if I need any help! Thanks for the offer.
Martin |
RE: Tissue Culture Propagation
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| Martin, if you don't mind I'd love to hear how it goes for you. Keep us posted if you have time, who knows, i might be able to help. What plant/plants are you trying? |
RE: Tissue Culture Propagation
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Hi Jamus,
Shall keep you informed. This was mainly an experiment to see how it works (and IF it works) - I have Banksia, Grevillea, Dendrobium orchid and Kangaroo paw, all from leaf tips. Would you agree that in that same order my expectations should be from least to most likely to shoot? I've heard that the woodier the plants the more difficult it is to get them to grow from TC. And that there has been some good results with Kangaroo Paw in the past...since its fairly soft.
It was suggested that I use 10:10:10 NPK soluble fertiliser in my medium, but I have chosen something with a lower P level for the natives. Was that a good idea or not?
I'm very much a beginner with propagation unfortunately, so I may be completely mad with my assumptions. Maybe its a bit early in my experiences to play around with TC and that I should learn a bit more about the technical side of horticulture before I attempt something such as this!! But I'm having fun, and thats why I do it :)
Thanks
Martin |
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